To explore the editing specificity of CRISPR/Cpf1 system, effects of target mutation were systematically examined using a reporter activation assay, with a set of single-nucleotide mutated target site ...

Based on engineered or bacterial nucleases, the development of genome editing technologies has opened up the possibility of directly targeting and modifying genomic sequences in almost all eukaryotic ...

Regions of the genome with repetitive sequences are difficult to selectively edit because it’s hard to control which of the repeated sections will be edited. Ribosomes, the molecular machines that ...

Proteins carry out exceptionally diverse molecular functions—even though they are all built on an unvarying polyamide backbone. How much more chemical diversity might be unlocked by editing that ...

Since the illnesses an individual experiences in a lifetime are largely determined by their genetics, there has been significant interest to better understand our genetic makeup for years. Our ...

Genome editing in vivo requires safe and efficient delivery of reagents to a target tissue. At the same time, delivery to non-target tissues, especially germline cells, must be assessed for potential ...

The CRISPR genome-editing technique revolutionising biology just got a major upgrade. A new variant, called prime editing, should be even better at correcting disease-causing mutations. This approach, ...