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4:07
YouTube
ENT surgery lecture by Dr Sunil Kumar Sharma
AUTOFLUORESCENCE&NARROW BAND IMAGING
1. Principle • Autofluorescence (AF): Detects natural tissue fluorescence emitted by endogenous fluorophores like collagen, elastin, and porphyrins when excited by blue/UV light (375–460 nm). • NBI: Uses specific blue and green wavelengths (415 nm & 540 nm) to enhance superficial mucosal vasculature visibility. 2. Diagnostic Target • AF ...
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2 months ago
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Two-photon fluorescence lifetime imaging microscopy of autofluorescence under cellular differentiation
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Pollen tube stained for mitochondria (MitoTracker Green, green) and lysosomes (Lysotracker Red, red). Watch the pollen tube extend from the crack in the pollen grain (visualized by its autofluorescence). Mitochondria don't quite advance to the very tip of the pollen tube but stop a few microns before the tip. Rendering of the data set was performed in arivis Vision4D®. Sample: courtesy of R. Whan, UNSW, Sydney, Australia. --- For more information on ZEISS Lattice Lightsheet 7 and further applica
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